A day in the lab

A day in the lab

Inoculating bacterial cultures

Today in the lab, I inoculated four flasks of bacterial cultures, which will be left overnight for the bacterial to clone themselves and produce multiple copies of a piece of DNA that I put into them...

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A day in the lab

Bacterial transformation

Today in the lab I did a bacterial transformation experiment, then left the bacteria to grow on agar plates overnight, so that they would clone themselves and produce multiple copies of a gene I am interested in...

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A day in the lab

Phase-contrast microscopy

One of the things I did today in the lab, was to check the health of some cells growing in culture, and to estimate their numbers because they need to be of a certain confluency before I can use them for any experiments...

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A day in the lab

PCR and gel electrophoresis to determine genotypes

Today in the lab I was doing genotyping. Samples of DNA were collected from the latest litters of the lab's colonies and their genotype had to be determined to check which of them carry genetic mutations in specific genes...

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A day in the lab

Purifying plasmid DNA from bacterial cultures

Today in the lab I was doing a miniprep experiment. This experiment allows the purification of plasmid DNA from bacterial cultures...

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A day in the lab

Dissecting neonatal brains to get glial cells

Today in the lab I was dissecting neonatal brains, in order to get glial cells and culture them for my experiments...

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A day in the lab

Inspecting microglia dissected out of neonatal brains

Today in the lab I was looking at brain microglia which were isolated from a mixed culture of both microglia and astrocytes three days earlier...

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Immunohistochemistry of brain slices and confocal microscopy

Today in the lab I was using the confocal microscope to look at different cell types on brain slices...

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Analysing the calcium activity of the white matter

Today in the lab, I was analysing the calcium activity of the white matter in the corpus callosum (the area of the brain that connects the two hemispheres)...

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Immunocytochemistry of cultured cells and confocal microscopy

Today in the lab I was using the confocal microscope to look at different cell types in culture...

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A day in the lab

Analysing the electrical activity of neurons using computer code

Today in the lab, I spent a lot of my time on the computer, analysing the electrical activity of neurons that I had previously recorded during my electrophysiology experiments...

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A day in the lab

Recording neuronal activity using electrophysiology equipment

Today in the lab I was doing an electrophysiology experiment, specifically known as the patch-clamp technique...

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A day in the lab

Histopathological analysis of brain slices following a stroke

Today in the lab I was analysing H&E stained brain slices to identify histopathological changes following a stroke...

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A day in the lab

Processing brain slices for microscopy

Today's experiment in the lab was all about staining brain slices with Hematoxylin & Eosin (H&E) for microscopy. In the timelapse below, you can see me performing the experiment, which lasted about 3.5 hours...

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A day in the lab

MRI image analysis to assess stroke damage

Today in the lab, I spent the entire time doing image analysis, using the MRI scans collected during an experiment that was testing possible new treatments for stroke...

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