Laboratory Skills

Competent in the following laboratory techniques

🧪 Bioinformatics Tools and Bioconductor R Packages

• Preprocessing: Nanoplot, NanoFilt, Guppy, FASTQC
• Alignment & Mapping: Samtools, minimap2, HTSeq, ClustalW
• Variant & Feature Analysis: IGV, seqtk, featureCounts, BEDTools
• Transcriptomics: DESeq2, Salmon, DRIMSeq, edgeR
• qPCR Normalisation: geNorm, NormqPCR
• Gene & Protein Interaction: STRING, Gene Ontology
• Functional Genomics & Pathway Analysis: clusterProfiler, GOplot
• Alternative Splicing: FLAIR, DEXSeq, SGSeq

⚙️ Machine Learning

• Dimensionality reduction, Principal Component Analysis
• Data classification, Hierarchical Clustering, k-Means clustering
• Regression trees, Gaussian process regression
• Time-series analysis, Signal interpolation
• Frequency analysis, Spectrum plotting, Sampling, Aliasing
• Feature extraction, Image segmentation
• Convolution filtering, Filter quality analysis

🧬 Molecular Biology and Biochemistry

• cDNA synthesis, PCR, qPCR, RT-qPCR
• RNA sequencing, microarrays
• DNA & RNA extraction
• Molecular cloning
• Gel electrophoresis, genotyping
• ELISA

🧫 Cellular Biology

• Primary and immortalised cell culture
• Transfection, viral transduction
• Cre-Lox recombination

🔬 Microscopy and Histology

• Histology
• IF, ICC, IHC
• Epifluorescence microscopy, confocal microscopy
• Vibratome slicing, cryo-sectioning

🐁 In vivo techniques

• Perfusion-fixation
• Intraperitoneal injections
• Inhalational anaesthesia
• Behavioural testing
• Muscle dissection