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Inoculating bacterial cultures

Today in the lab, I inoculated four flasks of bacterial cultures, which will be left overnight for the bacterial to clone themselves and produce multiple copies of a piece of DNA that I put into them. Bacterial cultures are grown in lysogeny broth, which contains peptides, vitamins, minerals, and a few other components, to promote optimum bacterial growth.

Timelapse: Preparing flasks with lysogeny broth, and inoculating them with bacteria.

Interesting facts about the timelapse video above

The glass barrier between me and the samples is part of a laminar flow cabinet which is used to prevent contamination of the samples. In laminar flow cabinets, air is drawn through a HEPA filter and blown in a laminar flow towards the user. Laminar flow simply means that the air flows with high momentum diffusion and low momentum convection. In simple words, air flow is very smooth and in layers with little or no mixing (as opposed to turbulent flow). Due to the direction of air flow, the sample is protected from the user but the user is not protected from the sample.