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Immunohistochemistry of brain slices and confocal microscopy

Today in the lab I was using the confocal microscope to look at different cell types on brain slices. Specifically, I was looking at glia, the cells that support neurons in the brain. Oligodendrocytes, which insulate neurons, were genetically engineered to express a green fluorescent protein after injection of tamoxifen. Myelin sheaths were stained with a blue fluorescent protein, and astrocytes were labelled with a red fluorescent protein, using a technique called immunohistochemistry, and specific antibodies that selectively bind to them.

Below, you can see some of the microscope images I collected.

Confocal images from a section of the corpus callosum in the adult brain, showing oligodendrocytes in green, myelin sheaths in blue, and astrocytes in red.
Confocal images from a section of the corpus callosum in the adult brain, showing oligodendrocytes in green, myelin sheaths in blue, and astrocytes in red.